Spontaneous Intestinal Tumorigenesis in Mice Requires Altered T Cell Development with IL-17A

Figure 4

Wild type inducible Tregs can regress intestinal tumors in mice. (a) Splenic naïve CD4 T cells from 6-7-week-old -FIR mice and their wild type littermate controls were isolated and differentiated into inducible Tregs (iTregs) for 120 hrs. A representative of three independent experiments is shown. . (b–d) Splenic naïve CD4 T cells from 6-7-week-old -FIR mice and their wild type littermate controls were isolated and differentiated into inducible Tregs (iTregs) and sorted. 4 × 10⁵ iTregs were transferred to 4 × 10⁵ ( for WT iTreg, for iTreg). Tumor numbers were measured 4 weeks after adoptive transfer. Data are shown as mean ± SEM. For (b–d), one-way ANOVA analysis with Bonferroni’s post hoc test was used. For other analyses, two-tailed -test was performed for all groups. ; ; ; n.s.: not significant. (e) LP cells from mice that received WT iTregs were compared with mice () and analyzed by flow cytometry.

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