Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Figure 5

Kinetics of leukocyte recruitment into the peritoneum after infection with A. salmonicida. (a) The total number of leukocytes in the peritoneal fluid in infected trout (filled bars) and PBS-injected controls (open bars) was determined with flow cytometry and is given as mean ± SEM from five fish per time point (ordinate). (b) Differentiation of the number of recruited cells into myeloid cells (square symbols) and lymphocytes (circles). (c) Alteration of the percentage of myeloid cells (square symbols) and lymphocytes (circles) after PBS injection (open symbols) or infection with A. salmonicida (filled symbols) as calculated from the data given in (b). Asterisks denote statistical significance with (), (), and (), compared to controls (0 hpi), assessed with one-way ANOVA, followed by Tukey’s post hoc test.

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(b)

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