Journal of Immunology Research / 2015 / Article / Tab 1 / Research Article
Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss )Table 1 Oligonucleotide primers used in this study.
Primer name Sense (3′-5′) Antisense (3′-5′) Primer efficiency Fragment length [bp] Tm [°C] Accession code TLR1_RT TGGAAATCCTCACGGCCAAG NM_001166101 TLR1_LC AAAGACAGAGATGAGGGCTGTG CGGTGCATGGAGGTAGGTTTC 2.04 161 85.5 TLR2_RT GTCATGGATGATAGAGGAGACGA HE979560 TLR2_LC GGAGAGGAGACCTGGCTGGA CCTGGAATGGTCCTCGGAGA 1.88 141 85.6 TLR3_RT CCAGGCCTTTGAAGGTGGTG NM_001124578 TLR3_LC AGTCCTCCACCTGTCGAATCTA GATCGCTGTCCCAGAAAGCTC 2.01 154 83.1 TLR5_RT TCCCCGTGCCCATATCATCT NM_001124744 TLR5_LC CAACTTCTTCGTTTGGCTGATAAT ACCAGAGAAACTCAATGTGCATTA 1.94 160 78.7 TLR7_RT AGCTGTAAAAACGCTCCCTCCTC GQ422119 TLR7_LC TGCACAGAGAGTGTGACTATCAA ATGTCAGAGAGGTTCGTTTTCAG 2.00 179 82.9 TLR8a1_RT GAAGGCACCTTCGGCAATGT GQ422121 TLR8a1_LC CTGTGTCACTTCCTGGTCATAAA TTTTCTGATAGGTCCAGCACAGT 2.05 187 82.5 TLR8a2_RT GAGCATTGGAGCATCGTGGA GQ422120 TLR8a2_LC TCTCGAATATGAGCAACCTTGTC AGTCCTTTTGGAACACGAGTTAAA 1.97 183 80.5 TLR9_RT TGCTCGTCAGCACAAACACG NM_001129991 TLR9_LC TGGTATTGCTTCCAGGTGCTGT CTCCAGGTGGACCAGCAGCT 2.01 155 88.0 TLR19_RT TCTCCAGGCCACACCAGTCA HF952170 TLR19_LC GAGAGGAGGAGGTGAGGTATG ATGCCCTTCCCTACCTCAAAGT 2.07 154 86.4 TLR20_RT GCCAGGGTGTGGTTCAGGAG DQ872849 TLR20_LC ACCCTCCGTCTGCTGGTGGA CCACAGACGGTCCCAGAAGG 1.87 174 87.3 TLR21_RT TCTCCCAGGTCCAGGTAGCAA CX261318 TLR21_LC CTGTTGAGGCAGGTGCAACTAA CTCAAAGGAGGTTTTTGGGAATC 2.06 156 83.2 TLR22a1_RT CGGGATAAATCCAACAGCCTCA NM_001124412 TLR22a1_LC TGGACAATGACGCTCTTTTACC GAGCTGATGGTTGCAATGAGG 2.02 151 83.6 TLR22a2_RT TCCCAATGGATTCCGGGATAA NM_001124419 TLR22a2_LC TAGAAGTCGTACCAAAAGACATTC GAGAGACCCATCATCCACTGAA 2.03 161 79.8 NOD1_RT Oligo(dT) CA372055 NOD1_LC CCGGTCAGCTGCTACGATGC AGTGCAGCAGGAGGGGCAAAA 2.02 160 85.0 MYD88_RT CCCCCTTCTGGGTCATCCTC NM_001124421 MYD88_LC GATCAAGAATTACGAGGATTGCC CCTCAATGAGAACTCGGAGATC 1.92 155 83.7 IRAK4a_RT TGAGGGATAGTCGTTCTTCCTGTTG FN598575 IRAK4a_LC GAGATTGATGAGGAGGAGATGG TCAAGCTCTGTCAGGACCTCTT 2.02 161 84.7 IRAK1_RT CTGCGACGGAGCAGCAAGT CA378286 IRAK1_LC ATGGACAGTATCTCCGATGTGG TGCAGCTTGTCCAGTACGTTCA 1.97 173 85.2 TRAF6_RT AAGCCCTTGGGGTTCCTCTG BX081400 TRAF6_LC CTCCAAACGCCCTCGGCACA CCACATGGTGCTGGCCCTCA 1.84 154 88.4 TOLLIP_RT CAGTGGGCACTCCTGCAATG NM_001124420 TOLLIP_LC CTCAGTGGCAGACAAGGCGA TCAGATATGGACATCTGCTTA 2.06 537 86.6 IL1B_RT TTCCACAGCACTCTCCAGCAA NM_001124347 IL1B_LC AAGTCTTTAAGCAACTGACTAAGC TGCACTTTCAGAGGTGTTCTTTAT 2.06 152 79.2 TNF_RT CTGACCTTACCCCGCTAAGAAGA NM_001124357 TNF_LC GATACCCACCATACATTGAAGCA ATTTGGTTCCCCTGTAGCTCGA 2.01 162 84.9 IL8_RT TTCCAACCTGGATAGAACATGATAGAA AJ279069 IL8_LC CTGAGGGGATGAGTCTGAGAG ATCTCCTGACCGCTCTTGCTC 2.05 162 85.3 IL10_RT GGTTTGAACAACAATGCGCAGA AB118099 IL10_LC CTCAGATGCGGTTTTCAAACACT CACAGTAAGCACTGGAACACAC 2.03 155 81.5 TGFB_RT CCTTGTGTTGTCTCCCCACATAAT X99303 TGFB_LC ATCAGGGATGAACAAGCTGAGG TTCGCACACAGCAACTCTCCG 1.72 182 83.5 IgM_RT GAGCTGACTGTAGACAGAGTAG S63348 IgM_LC TACAAGAGGGAGACCGGAGGA CACCGGCTCATCGTCAACAAG 1.92 159 84,5 TRB_RT ATGAAGATGCTGTAGGCCAGCT EU072699 TRB_LC GTCTTCTGGCAAGTCAACAATGT GTAAAAGCTGACAATGCAGGTGA 1.94 165 83.7
