Journal of Immunology Research

Research Article

Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Table 3

(a) Expression profiles of various PRRs in selected tissues of rainbow trout at different time points after infection. (b) Expression profiles of various downstream factors in selected tissues of rainbow trout at different time points after infection.

(a)


Gene symbol	Tissues	Fold change values at time points after infection relative to control
Gene symbol	Tissues	6 h	12 h	24 h	48 h	72 h

tlr1	Spleen	−1.07 ± 0.19	−1.02 ± 0.23	1.07 ± 0.16	−1.07 ± 0.18	−1.54 ± 0.28
	Head kidney	1.61 ± 0.18	1.72 ± 0.25	1.50 ± 0.27	1.57 ± 0.21	1.66 ± 0.13
	Liver	−1.11 ± 0.18	1.72 ± 0.31	2.32 ± 0.56	1.67 ± 0.31	1.70 ± 0.22
	Thymus	−1.31 ± 0.29	−1.20 ± 0.32	1.21 ± 0.29	1.13 ± 0.24	1.09 ± 0.20

tlr2	Spleen	−1.13 ± 0.16	−1.73 ± 0.33	−1.86 ± 0.41	−1.46 ± 0.15	−1.68 ± 0.20
	Head kidney	1.24 ± 0.19	−1.13 ± 0.17	−1.68 ± 0.28	−1.10 ± 0.22	−1.18 ± 0.15
	Liver	−1.01 ± 0.11	−1.11 ± 0.11	−1.01 ± 0.12	1.50 ± 0.32	1.16 ± 0.18
	Thymus	−1.12 ± 0.21	−1.07 ± 0.19	−1.07 ± 0.25	1.16 ± 0.19	1.22 ± 0.16

tlr3	Spleen	−1.58 ± 0.17	−1.67 ± 0.25	−1.21 ± 0.25	−1.82 ± 0.23	−1.70 ± 0.23
	Head kidney	−1.52 ± 0.17	−1.99 ± 0.26	−2.49 ± 0.30	−2.08 ± 0.29	−2.09 ± 0.32
	Liver	−1.21 ± 0.31	−1.64 ± 0.39	−1.40 ± 0.36	−1.83 ± 0.47	−1.64 ± 0.51
	Thymus	−1.26 ± 0.27	−1.87 ± 0.49	−1.46 ± 0.37	−1.52 ± 0.37	−1.63 ± 0.34

tlr5	Spleen	−2.77 ± 0.71	−3.88 ± 0.71	−2.76 ± 0.70	−2.34 ± 0.66	−1.61 ± 0.49
	Head kidney	−1.07 ± 0.27	1.03 ± 0.64	−2.33 ± 0.48	−1.62 ± 0.47	−1.55 ± 0.34
	Liver	1.28 ± 0.31	−1.30 ± 0.25	1.77 ± 0.37	1.99 ± 0.37	1.96 ± 0.38
	Thymus	1.14 ± 0.23	−1.12 ± 0.29	−2.08 ± 0.35	−1.29 ± 0.30	−1.91 ± 0.52

tlr7	Spleen	−2.10 ± 0.24	−1.62 ± 0.58	−2.79 ± 0.48	−3.23 ± 0.62	−3.40 ± 0.85
	Head kidney	1.95 ± 0.45	2.22 ± 0.63	1.51 ± 0.49	−1.28 ± 0.35	−1.04 ± 0.24
	Liver	−1.01 ± 0.23	1.06 ± 0.19	1.17 ± 0.20	1.33 ± 0.41	−1.05 ± 0.30
	Thymus	−1.30 ± 0.28	−1.34 ± 0.33	−1.03 ± 0.40	−1.35 ± 0.28	−1.47 ± 0.31

tlr8a1	Spleen	−2.03 ± 0.29	−1.76 ± 0.28	−1.29 ± 0.20	−1.36 ± 0.23	−1.62 ± 0.29
	Head kidney	−1.35 ± 0.22	−1.89 ± 0.39	−1.90 ± 0.34	−1.30 ± 0.45	−1.89 ± 0.77
	Liver	1.47 ± 0.41	1.81 ± 0.42	2.86 ± 0.77	2.88 ± 1.00	1.67 ± 0.43
	Thymus	1.02 ± 0.21	−1.01 ± 0.27	−1.09 ± 0.28	1.15 ± 0.28	−1.41 ± 0.30

tlr8a2	Spleen	−2.45 ± 0.53	−1.99 ± 0.29	−1.49 ± 0.27	−1.85 ± 0.32	−2.75 ± 0.45
	Head kidney	−1.31 ± 0.43	−1.46 ± 0.31	−1.23 ± 0.24	−1.29 ± 0.27	−1.91 ± 0.31
	Liver	1.54 ± 0.50	1.75 ± 0.56	2.30 ± 0.56	2.56 ± 0.81	1.54 ± 0.42
	Thymus	1.07 ± 0.27	−1.49 ± 0.33	−1.27 ± 0.32	1.03 ± 0.45	−1.28 ± 0.47

tlr9	Spleen	1.09 ± 0.11	1.32 ± 0.11	1.44 ± 0.17	1.20 ± 0.22	−2.00 ± 0.25
	Head kidney	1.33 ± 0.19	1.35 ± 0.25	1.24 ± 0.21	1.29 ± 0.18	1.43 ± 0.17
	Liver	1.50 ± 0.22	1.95 ± 0.32	2.57 ± 0.42	2.87 ± 0.52	3.44 ± 0.51
	Thymus	−1.22 ± 0.37	−1.20 ± 0.40	1.15 ± 0.26	1.20 ± 0.39	1.33 ± 0.42

tlr19	Spleen	−1.22 ± 0.28	−1.66 ± 0.59	−1.62 ± 0.46	1.45 ± 0.38	−2.82 ± 0.66
	Head kidney	3.18 ± 0.84	2.52 ± 0.54	2.73 ± 0.85	2.19 ± 0.64	2.00 ± 0.55
	Liver	2.83 ± 1.53	−1.82 ± 0.59	1.00 ± 0.23	−1.90 ± 0.54	−2.00 ± 0.57
	Thymus	1.06 ± 0.39	−1.22 ± 0.37	1.35 ± 0.55	1.02 ± 0.30	1.42 ± 0.43

tlr20	Spleen	−1.58 ± 0.29	−1.71 ± 0.38	−1.23 ± 0.21	1.08 ± 0.27	−1.02 ± 0.25
	Head kidney	1.29 ± 0.20	−1.40 ± 0.21	1.07 ± 0.17	1.24 ± 0.18	1.49 ± 0.21
	Liver	1.55 ± 0.23	1.74 ± 0.20	2.01 ± 0.19	2.20 ± 0.25	2.02 ± 0.26
	Thymus	−1.17 ± 0.24	1.32 ± 0.35	−1.22 ± 0.32	1.19 ± 0.26	1.42 ± 0.31

tlr21	Spleen	−1.39 ± 0.28	−3.82 ± 0.46	−1.83 ± 0.40	−1.25 ± 0.35	−1.03 ± 0.19
	Head kidney	−1.06 ± 0.26	−2.96 ± 0.61	−3.36 ± 0.51	−1.65 ± 0.26	−1.52 ± 0.24
	Liver	1.67 ± 0.48	1.61 ± 0.44	1.34 ± 0.21	2.16 ± 0.58	1.51 ± 0.34
	Thymus	1.07 ± 0.18	−1.31 ± 0.26	−1.47 ± 0.29	1.00 ± 0.21	−1.13 ± 0.21

tlr22a1	Spleen	1.21 ± 0.27	−1.18 ± 0.22	−1.10 ± 0.23	1.01 ± 0.27	−1.99 ± 0.45
	Head kidney	1.55 ± 0.50	1.41 ± 0.34	1.51 ± 0.56	1.06 ± 0.24	−1.75 ± 0.44
	Liver	2.12 ± 0.52	1.94 ± 0.43	2.50 ± 0.58	3.23 ± 1.00	1.41 ± 0.34
	Thymus	1.24 ± 0.21	−1.11 ± 0.29	1.11 ± 0.35	1.07 ± 0.23	−1.46 ± 0.23

tlr22a2	Spleen	3.02 ± 0.64	1.21 ± 0.24	1.11 ± 0.11	1.89 ± 0.39	1.53 ± 0.30
	Head kidney	6.14 ± 2.37	3.81 ± 0.70	1.63 ± 0.32	3.27 ± 0.70	2.71 ± 0.48
	Liver	3.51 ± 0.79	4.41 ± 0.97	2.94 ± 0.85	3.85 ± 0.97	3.12 ± 0.80
	Thymus	1.37 ± 0.25	1.83 ± 0.33	1.48 ± 0.40	1.23 ± 0.34	1.15 ± 0.28

nod1	Spleen	−1.05 ± 0.15	−1.60 ± 0.20	−1.01 ± 0.14	1.07 ± 0.12	−1.13 ± 0.13
	Head kidney	−1.22 ± 0.23	−3.08 ± 0.61	−1.60 ± 0.26	−1.31 ± 0.32	−1.59 ± 0.35
	Liver	1.20 ± 0.18	1.26 ± 0.19	1.37 ± 0.19	1.36 ± 0.30	1.74 ± 0.34
	Thymus	−1.02 ± 0.21	1.45 ± 0.33	1.35 ± 0.39	−1.04 ± 0.27	−1.15 ± 0.26

Significant expression difference () between the infected and the control groups is indicated in bold.

(b)


Gene symbol	Tissues	Fold change values of infected group relative to the control
Gene symbol	Tissues	6 h	12 h	24 h	48 h	72 h

myd88	Spleen	1.44 ± 0.25	1.68 ± 0.24	1.33 ± 0.33	1.36 ± 0.22	1.25 ± 0.20
	Head kidney	1.37 ± 0.16	1.33 ± 0.20	1.22 ± 0.22	1.45 ± 0.29	1.04 ± 0.12
	Liver	2.75 ± 0.48	6.68 ± 0.63	3.34 ± 0.76	4.37 ± 1.48	6.78 ± 1.89
	Thymus	1.08 ± 0.14	−1.13 ± 0.23	1.01 ± 0.21	1.09 ± 0.17	1.00 ± 0.14

irak4a	Spleen	−1.02 ± 0.14	−1.20 ± 0.10	1.26 ± 0.09	1.03 ± 0.16	−1.06 ± 0.14
	Head kidney	1.04 ± 0.11	−1.25 ± 0.16	−1.36 ± 0.19	−1.31 ± 0.18	−1.39 ± 0.19
	Liver	1.38 ± 0.14	1.67 ± 0.14	1.87 ± 0.24	1.51 ± 0.15	1.86 ± 0.23
	Thymus	1.17 ± 0.16	1.10 ± 0.18	1.08 ± 0.18	1.26 ± 0.17	1.71 ± 0.23

irak1	Spleen	2.81 ± 0.43	2.86 ± 0.24	1.56 ± 0.17	1.51 ± 0.22	1.13 ± 0.20
	Head kidney	2.24 ± 0.35	2.54 ± 0.47	1.23 ± 0.15	1.34 ± 0.17	1.55 ± 0.18
	Liver	2.60 ± 0.50	3.93 ± 0.49	2.12 ± 0.35	2.14 ± 0.30	2.34 ± 0.34
	Thymus	1.11 ± 0.13	−1.07 ± 0.19	−1.25 ± 0.21	−1.14 ± 0.16	1.13 ± 0.15

traf6	Spleen	1.10 ± 0.15	1.29 ± 0.13	1.26 ± 0.13	1.21 ± 0.16	1.15 ± 0.13
	Head kidney	1.05 ± 0.09	1.49 ± 0.15	1.36 ± 0.14	1.57 ± 0.15	1.37 ± 0.15
	Liver	1.27 ± 0.09	2.21 ± 0.27	2.21 ± 0.30	2.04 ± 0.33	2.17 ± 0.25
	Thymus	1.18 ± 0.15	−1.04 ± 0.11	−1.01 ± 0.15	−1.03 ± 0.13	1.78 ± 0.22

tollip	Spleen	1.06 ± 0.19	1.21 ± 0.21	1.29 ± 0.22	1.15 ± 0.23	1.24 ± 0.21
	Head kidney	1.31 ± 0.21	−1.05 ± 0.24	1.14 ± 0.12	1.27 ± 0.14	1.11 ± 0.10
	Liver	1.62 ± 0.48	1.73 ± 0.54	2.74 ± 0.87	3.44 ± 1.14	4.53 ± 1.43
	Thymus	1.03 ± 0.15	−1.11 ± 0.29	1.05 ± 0.24	−1.04 ± 0.19	1.29 ± 0.26

Significant expression difference () between the infected and the control groups is indicated in bold.