Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies

Table 2

Qualitative and quantitative agreements between anti-dsDNA antibody assays.

	NOVA Lite; dsDNA CLIFT	QUANTA Flash; dsDNA	QUANTA Lite; dsDNA SC	BioPlex 2200; dsDNA	ImmuLisa; dsDNA
QUANTA Flash; dsDNA	85.7 (79.3–90.7); kappa = 0.26; (0.08–0.45)		78.9 (71.8–84.9); kappa = 0.39; (0.22–0.55)	80.7 (73.8–86.5); kappa = 0.42; (0.25–0.59)	83.9 (77.2–89.2); kappa = 0.37; (0.17–0.56)
	N/A^*		rho = 0.58; (0.46–0.67); P < 0.0001	rho = 0.46; (0.33–0.57); P < 0.0001	rho = 0.34; (0.19–0.47); P < 0.0001
QUANTA Lite; dsDNA SC	77.0 (69.7–83.3); kappa = 0.17; (0.04–0.30)	78.9 (71.8–84.9); kappa = 0.39; (0.22–0.55)		78.3 (71.1–84.4); kappa = 0.42; (0.26–0.58)	80.1 (73.1–86.0); kappa = 0.38; (0.22–0.54)
	N/A^*	rho = 0.58; (0.46–0.67); P < 0.0001		rho = 0.43; (0.30–0.55); P < 0.0001	rho = 0.39; (0.25–0.51); P < 0.0001
BioPlex 2200; dsDNA	78.9 (71.8–84.9); kappa = 0.18; (0.04–0.32)	80.7 (73.8–86.5); kappa = 0.42; (0.25–0.59)	78.3 (71.1–84.4); kappa = 0.42; (0.26–0.58)		80.7 (73.8–86.5); kappa = 0.37; (0.20–0.54)
	N/A^*	rho = 0.46; (0.33–0.57); P < 0.0001	rho = 0.43; (0.30–0.55); P < 0.0001		rho = 0.30; (0.16–0.44); P < 0.0001
ImmuLisa; dsDNA	89.4% (83.6–93.7); kappa = 0.28; (0.05–0.51)	83.9 (77.2–89.2)  kappa = 0.37; (0.17–0.56)	80.1 (73.1–86.0); kappa = 0.38; (0.22–0.54)	80.7 (73.8–86.5 ); kappa = 0.37; (0.20–0.54)
	N/A^*	rho = 0.34; (0.19–0.47); P < 0.0001	rho = 0.39; (0.25–0.51); P < 0.0001	rho = 0.30; (0.16–0.44); P < 0.0001

Note: total qualitative agreements are given in percent, followed by kappa statistics and Spearman’s rho values (95% confidence intervals are provided in the parentheses). ^*NOVA Lite CLIFT was excluded from the analysis since it is a semiquantitative assay where grading values (0 to 4) are given by the operator.