Journal of Immunology Research / 2015 / Article / Tab 2

Research Article

Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies

Table 2

Qualitative and quantitative agreements between anti-dsDNA antibody assays.

NOVA Lite  
dsDNA CLIFT
QUANTA Flash 
dsDNA
QUANTA Lite  
dsDNA SC
BioPlex 2200 
dsDNA
ImmuLisa 
dsDNA

QUANTA Flash   
dsDNA
85.7 (79.3–90.7)  
kappa = 0.26  
(0.08–0.45)  
78.9 (71.8–84.9)  
kappa = 0.39  
(0.22–0.55)  
80.7 (73.8–86.5)  
kappa = 0.42  
(0.25–0.59)  
83.9 (77.2–89.2)  
kappa = 0.37  
(0.17–0.56)  
N/A*rho = 0.58
(0.46–0.67)
P < 0.0001
rho = 0.46
(0.33–0.57)
P < 0.0001
rho = 0.34
(0.19–0.47)
P < 0.0001

QUANTA Lite  
dsDNA SC
77.0 (69.7–83.3)  
kappa = 0.17  
(0.04–0.30)  
78.9 (71.8–84.9)
kappa = 0.39
(0.22–0.55)
78.3 (71.1–84.4)  
kappa = 0.42  
(0.26–0.58)  
80.1 (73.1–86.0)  
kappa = 0.38  
(0.22–0.54)  
N/A*rho = 0.58
(0.46–0.67)
P < 0.0001
rho = 0.43
(0.30–0.55)  
P < 0.0001
rho = 0.39  
(0.25–0.51)  
P < 0.0001

BioPlex 2200  
dsDNA
78.9 (71.8–84.9)  
kappa = 0.18  
(0.04–0.32)  
80.7 (73.8–86.5)
kappa = 0.42
(0.25–0.59)
78.3 (71.1–84.4)
kappa = 0.42
(0.26–0.58)
80.7 (73.8–86.5)
kappa = 0.37  
(0.20–0.54)  
N/A*rho = 0.46
(0.33–0.57)
P < 0.0001
rho = 0.43
(0.30–0.55)
P < 0.0001
rho = 0.30  
(0.16–0.44)  
P < 0.0001

ImmuLisa  
dsDNA
89.4% (83.6–93.7)  
kappa = 0.28  
(0.05–0.51)  
83.9 (77.2–89.2)  kappa = 0.37  
(0.17–0.56)  
80.1 (73.1–86.0)  
kappa = 0.38  
(0.22–0.54)  
80.7 (73.8–86.5 )
kappa = 0.37  
(0.20–0.54)  
N/A*rho = 0.34  
(0.19–0.47)  
P < 0.0001
rho = 0.39  
(0.25–0.51)  
P < 0.0001
rho = 0.30  
(0.16–0.44)  
P < 0.0001

Note: total qualitative agreements are given in percent, followed by kappa statistics and Spearman’s rho values (95% confidence intervals are provided in the parentheses). *NOVA Lite CLIFT was excluded from the analysis since it is a semiquantitative assay where grading values (0 to 4) are given by the operator.