Application of “Systems Vaccinology” to Evaluate Inflammation and Reactogenicity of Adjuvanted Preventative Vaccines

Table 2

Harmonized minimum data set of immune parameters for cross species evaluation of responses to adjuvanted vaccines and TLR agonists.

Vaccine or TLR agonist	Commercially available Luminex assays	Bespoke Luminex assays to mirror clinical studies	Bespoke qPCR plasmid calibrants for ferrets
Pentavalent/whole cell pertussis	IL-1	C-reactive protein	IL1
Trivalent influenza	IL-1	Serum amyloid A	IL6
Trivalent influenza + MF59	IL-2	a2-macroglobulin	IL8
Engerix B	IL-3	LPS binding protein	CXCL10
Varicella vaccine	IL-4	Procalcitonin	CCL2
Poly I: C	IL-5	PTX3 pentraxin	IFN
Lipopolysaccharide	IL-6	TREM-1	TNF
Incomplete Freund’s adjuvant	IL-9		Serum amyloid A
	IL-10		GAPDH
	IL-12 (p40)		RPL32
	IL-12 (p70)
	IL-13
	IL-17A
	KC (CXCL1)
	MCP-1 (CCL2)
	MIP-1 (CCL3)
	MIP-1 (CCL4)
	RANTES
	IFN-
	TNF-
	GM-CSF
	G-CSF
	Eotaxin