Lactobacillus acidophilus Suppresses Colitis-Associated Activation of the IL-23/Th17 Axis
Oral administration of Lactobacillus acidophilus inhibited IL-17 expression in DSS-induced colitis. The mRNA (a) and protein (b) levels of IL-17 expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated groups C4–C8 (oral gavage of 104, 105, 106, 107, or 108 CFU/10 g body weight, resp.), prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD () values are shown on the right. A plotted trendline chart shows IL-17 expression in each group at both mRNA and protein levels (c). versus Non, versus Veh, versus Pred, and versus normal.