HMGB1 Promotes Systemic Lupus Erythematosus by Enhancing Macrophage Inflammatory Response

Figure 2

HMGB1 overexpression could promote the severity of SLE. BALB/c mice were administrated intramuscularly with 100 μg pHMGB1 or empty-vector per mouse. 72 h following injection, mice were then injected subcutaneously with ALD-DNA (50 μg/mouse) for total 3 times in 4 weeks. (a) The dynamics of serum HMGB1 levels were determined by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (b) Nephritic pathology was evaluated by H&E staining of renal tissues. Images (magnification ×200) are representative of at least 8 mice in each group. (c) The kidney score was assessed using paraffin sections stained with H&E in (b). (). (d) Serum anti-dsDNA antibody levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (e) Urine protein levels of the mice were assessed by BCA method every 2 weeks. Data are means ± SD from 8 mice in each group. .

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