Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells

Figure 1

MelanA is expressed in cocktail-matured, monocyte-derived DCs after mRNA electroporation. DCs were either electroporated without mRNA (mock; gray histogram) or with mRNA encoding the tumor antigen MelanA (MelanA; black histogram). (a) Surface marker expression of CD25, CD40, CD83, CD86, CD70, and CD80 on mock-electroporated and MelanA-RNA-electroporated DCs 24 h after electroporation is shown (black lines; respective isotype controls). One representative of ≥4 experiments is shown. (b) Gating of mock-electroporated (Mock) or MelanA-RNA-electroporated (MelanA) DCs was performed according to forward and side scatter. (c) Four and twenty-four hours after electroporation, the intracellular MelanA expression was determined by flow cytometry. The percentage of positive cells is indicated. One representative of >10 experiments is shown.

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