Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells

Figure 4

Massive cytokine secretion after electroporation with mRNA encoding a DC-activating protein. DCs were electroporated either without RNA (Mock) or with RNA encoding the tumor antigen MelanA (MelanA) or MelanA and constitutively active stabilized mutated IKKβ. Twenty-four hours after electroporation, supernatants of the cells were taken and the cytokine secretion by the cells was determined in a cytometric bead array. The cytokine concentrations in the supernatants are depicted. Each symbol represents an individual donor, tested in an independent experiment (). The horizontal bars show the average values. values were calculated by the 1-way ANOVA with multiple comparison test (according to Tukey) with a confidence level of 0.05; ns: , , and .