Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells

Table 1

mRNAs used for transfection.

Antigen	Description	Abbreviation
MelanA	MelanA (MART1) full length wild type	MelanA
NRAS	NRAS-fragment 40 AA around mutation site 61 with DC-LAMP signal and flag-tag	NRAS-DCL
NRAS Q61K	NRAS-fragment 40 AA around mutation Q61K with DC-LAMP signal and flag-tag	NRAS-DCL K
NRAS Q61R	NRAS-fragment 40 AA around mutation Q61R with DC-LAMP signal and flag-tag	NRAS-DCL R
BRAF	BRAF-fragment 67 AA around mutation site 600 with flag-tag	BRAF
BRAF V600E	BRAF-fragment 67 AA around mutation V600E with flag-tag	BRAF E
BRAF	BRAF-fragment 67 AA around mutation site 600 with DC-LAMP signal and flag-tag	BRAF-DCL
BRAF V600E	BRAF-fragment 67 AA around mutation V600E with DC-LAMP signal and flag-tag	BRAF-DCL E
GNAQ	GNAQ-fragment 47 AA around mutation site 209 with DC-LAMP signal and flag-tag	GQ-DCL
GNAQ Q209P	GNAQ-fragment 47 AA around mutation Q209P with DC-LAMP signal and flag-tag	GQ-DCL P
GNAQ Q209L	GNAQ-fragment 47 AA around mutation Q209L with DC-LAMP signal and flag-tag	GQ-DCL L
GNA11	GNA11-fragment 47 AA around mutation site 209 with DC-LAMP signal and flag-tag	G11-DCL
GNA11 Q209P	GNA11-fragment 47 AA around mutation Q209P with DC-LAMP signal and flag-tag	G11-DCL P
GNA11 Q209L	GNA11-fragment 47 AA around mutation Q209L with DC-LAMP signal and flag-tag	G11-DCL L
WT-1	Wilms tumor 1 full length wild type	WT1
GNAQ	Wilms tumor 1 full length with DC-LAMP signal and flag-tag	WT1-DCL
	Constitutively active stabilized IKKβ to activate NF-κB	IKKβ

Not a tumor antigen, but a DC-activating protein.