A Review of Intra- and Extracellular Antigen Delivery Systems for Virus Vaccines of Finfish

Figure 2

The fusion of the VP2- of IPNV to the Pseudomonas aeruginosa exotoxin A (PE). PE shows a 602 aa exotoxin for Pseudomonas aeruginosa made of three domains. Domain Ia (blue) located on 1–252 aa followed by domain II (green) on the location 253–364 aa, domain Ib (blue) located on 365–404 aa, and finally domain III on the extreme end located on 405–601 aa. PJJ9 (ΔIII) is a 425 aa long intermediate phase in which the toxic moiety of domain III has been cleaved. PE-VP2-KE is the final construct in which the toxic moiety of domain III has been replaced with the truncated VP2 (yellow) immunogenic protein of infectious pancreatic necrosis virus (IPNV). Note that the VP2 is attached to the KDEL3 signaling peptide (red).