Exposure of Monocytes to Lipoarabinomannan Promotes Their Differentiation into Functionally and Phenotypically Immature Macrophages

Figure 8

Monocytes exposed to LAM are differentiated into functionally deficient macrophages. CD14⁺ monocytes incubated with LAM for 24–120 h at 37°C. On day 7 cells were stimulated with LPS (1 μg/mL) for 24 h. TNFα, IL-1β, and IFN-γ in the supernatant were measured by ELISA (a). Phagocytosis of latex beads was analyzed on the exposed MDM. Data are representative of five independent experiments in (a) or two independent experiments in (b). Box plot indicates median ± IQR (5–95). , . Kruskal-Wallis and Dunn post hoc tests compared to unexposed MDM.

(a)

(b)