TNF-α Autocrine Feedback Loops in Human Monocytes: The Pro- and Anti-Inflammatory Roles of the TNF-α Receptors Support the Concept of Selective TNFR1 Blockade In Vivo

Figure 2

Effect of selective TNF-α receptor blockade on cytokine mRNA expression. Monocytes were stimulated with 100 ng/mL of LPS (positive control) for 20 hours ± TNFR1/TNFR2 mAb/both (10 μg/mL), and cytokine mRNA expression was measured. Each column shows median (IQR) values. Overall differences between experimental conditions for each cytokine were assessed with Friedman’s test and post hoc pairwise comparisons conducted if appropriate (post hoc values are shown in the figures). (a) TNF-α: no overall difference observed. (b) CXCL8: Friedman’s test . (c) IL-1β: Friedman’s test . (d) IL-6: no overall difference observed. (e) IL-10: Friedman’s test . The experiments represent data from 7 subjects. Experimental conditions were conducted singly in culture wells and the resultant cellular material used in duplicate for subsequent real-time PCR.

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