Low-Dose Oxygen Enhances Macrophage-Derived Bacterial Clearance following Cigarette Smoke Exposure

Figure 4

Oxygen modulation of macrophage phenotype may support PAO1 phagocytosis. (a) Following in vitro CS, oxygen, and PAO1 exposure, we measured TNF-α in the supernatant (, mean ± SEM, by one-way ANOVA compared to both 21% oxygen-exposed groups). (b) Following in vitro CS, oxygen, and PAO1-GFP exposure, we measured the MFI of cell surface expression of macrophage markers CD86, Dectin-1, and MMR using flow cytometry and expressed values normalized to control across trials (mean ± SEM, , compared to control + 40% O₂ group by one-way ANOVA with Bonferroni correction). (c) Using FACS, we demonstrate scatter plots of macrophage GFP MFI expression (to denote PAO1 macrophage binding or engulfment) with macrophage MFI expression for Dectin-1 (), CD86 (), and MMR () surface markers.

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