Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response

Figure 2

Anti-DT serum blocking. (a) Representative images of Foxp3⁺ T cells within CD4⁺ T cells with/without anti-DT serum blocking. (b) Frequency of Foxp3⁺ T cells within CD4⁺ T cells with/without anti-DT serum blocking. Six wild type C57BL6 mice were injected 110 ng DT i.p. 3 times per week and maintained for another 4 weeks. Mice were sacrificed and blood was taken from heart puncture. Serum anti-DT antibody levels were detected by ELISA. Seropositive samples were pooled, and from this pool 200 μL anti-DT serum was injected i.p. per mouse, and subsequently 110 ng DT/mouse was injected i.p. one day later. All the animals were sacrificed at the 3rd day, and CD4⁺Foxp3⁺ frequency in the spleen was measured by flow cytometry. .

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