Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish

Table 2

Primer sequences used for amplification of specific genes through RT-qPCR.

Gene	Forward primer	Reverse primer	Amplicon (bp)	Gene ID	Efficiency
muc2.2	ACACGCTCAAGTAATCGCACAGTC	TCAGCGAGTGTTTGGCTCACTT	137	XM_002667543	1.82
mmp9	CATTAAAGATGCCCTGATGTATCCC	AGTGGTGGTCCGTGGTTGAG	142	NM_213123.1	1.89
β-def-1	CTCCTTGTCGTACTAGCATTGCAC	ACACACTCCTTGTCTGCAAACACC	99	NM_001081553.1	1.86
fabp2	TCAACGGGACCTGGAAAGTC	CCCATTTGTTCCATGAACTTCTC	61	NM_131431.1	1.86
fabp6	CTCCGCTCAATCAACACCAA	TGAGATTCGGTTTCCCACTTG	59	NM_001002076.1	1.93
β-actin	TTCTGGTCGTACTACTGGTATTGTG	ATCTTCATCAGGTAGTCTGTCAGGT	144	NM_131031.1	1.99
rpl13α	TCTGGAGGACTGTAAGAGGTATGC	AGACGCACAATCTTGAGAGCAG	148	NM_212784.1	1.94

Primers for mmp9, muc2.2, β-def-1, fapb2, and fabp6 were designed using AmplifX v1.4.0. Two reference genes, β-actin and ribosomal protein L13a (rpl13α), were used [10].