Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)

Figure 1

Cloning of OfPPO1a and OfPPO1b. (a) Schematic demonstration of the process for cloning full-length OfPPO1a (NCBI accession number: KX452359) and OfPPO1b (NCBI accession number: KX437622). The fragments with identical nucleotide sequences are illustrated in lines with the same color. The specific primers are labeled with the name followed by its position in the located fragment and indicated by the arrows above or below the lines. The predicted start codon and stop codon are indicated at the corresponding positions. (b) Comparisons and sequence analysis of deduced OfPPO1a and OfPPO1b. The deduced amino acid residues are shown in the same color scheme as in part (a). Identical residues in PPO1a and PPO1b are substituted by hyphens in PPO1b. Regions common in PPO1a and PPO1b are indicated by an irregular rectangle. The predicted proteolytic cleavage bonds were shown in red and marked with black arrow. The potential copper binding site A (CuA), copper binding site B (CuB), the putative thiol ester sites, and a conserved motif at the C-terminus were shaded in yellow, grey, green, and purple, respectively. The six histidine residues absolutely conserved within the CuA and CuB were in the square boxes. Two predicted disulfide linkages are represented by dashed lines.

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