Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)

Figure 2

Nucleotide and amino acid sequence of O. furnacalis PPO3. The deduced amino acid sequences are shown below the nucleotide sequence of PPO3 (NCBI accession number: KX437621). Nucleotides are numbered from the first base at the 5′-end. Amino acid residues were numbered from the initiating methionine. The one-letter code for each amino acid is aligned with the second nucleotide of the corresponding codon. The stop codon is marked with asterisk. The cDNA fragment from 3′-RACE started from the dashed-line arrow to the end. The predicted proteolytic cleavage bonds are shown in red and marked with red arrow. The potential copper binding site A (CuA), copper binding site B (CuB), the putative thiol ester sites, and a conserved motif at the C-terminus are shaded in yellow, grey, green, and purple, respectively. The six histidine residues absolutely conserved within the CuA and CuB are in the square boxes. Two predicted disulfide linkages are represented by dashed lines.