Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)

Figure 5

RT-PCR analysis of the expression of four O. furnacalis PPOs. (a) Expression profiles of OfPPOs at different stages of development. RNA was extracted from the whole bodies collected from eggs, first-instar (L1), second-instar (L2), third-instar (L3), fourth-instar (L4), fifth-instar (L5) larvae, and pupae. The rpL8 was used as an internal control. (b) Expression patterns of OfPPOs in different tissues of O. furnacalis larvae. Tissues including head, gut, hemocytes (HC), and fat bodies (FB) were collected from day-zero, fifth-instar larvae for RNA extraction. RT-PCR was performed to assess the transcript level of OfPPOs. The rpL8 was used to normalize the templates. (c) Expression profiles of PPOs in O. furnacalis larvae upon microbial challenge. Day 1, fifth-instar larvae were infected with water, E. coli, M. luteus, or B. bassiana. RNA was prepared from the whole bodies 24 h after injection. RT-PCR was used to analyze the transcript change of OfPPOs. The rpL8 was used as an internal standard to indicate a consistent total mRNA amount.

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