F4/80⁺ Host Macrophages Are a Barrier to Murine Embryonic Stem Cell-Derived Hematopoietic Progenitor Engraftment In Vivo

Figure 4

NSG macrophages preferentially phagocytose ESHP in vitro. (a) Representative contour plots of CFSE-labeled targets (ESHP or 129 Lin⁻ BM) internalized by NSG F4/80⁺ macrophages in vitro. (b) Quantification of targets internalized by F4/80⁺ macrophages. 129 macrophages alone (), 129 macrophages with syngeneic 129 Lin⁻ BM (), 129 macrophages with syngeneic ESHP (), NSG macrophages alone (), NSG macrophages with allogeneic Lin⁻ BM (), and NSG macrophages with allogeneic ESHP (). Bars represent mean ± SEM; ; ; .

(a)

(b)