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Journal of Immunology Research
Volume 2016, Article ID 2414906, 12 pages
http://dx.doi.org/10.1155/2016/2414906
Research Article

F4/80+ Host Macrophages Are a Barrier to Murine Embryonic Stem Cell-Derived Hematopoietic Progenitor Engraftment In Vivo

1Quantitative and Systems Biology Graduate Group, School of Natural Sciences, University of California-Merced, Merced, CA 95340, USA
2Department of Molecular Cell Biology, Vrije University, Amsterdam, Netherlands
3Molecular and Cell Biology Unit, School of Natural Sciences, University of California-Merced, Merced, CA 95340, USA

Received 22 May 2016; Revised 3 September 2016; Accepted 4 October 2016

Academic Editor: Xiao-Feng Yang

Copyright © 2016 Heather L. Thompson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: ESHPs are capable of multi-lineage development in vivo.

Supplementary Figure 2: ESHPs reconstitute the thymus of NSG mice.

Supplementary Figure 3: Percentages of host myeloid cells in the spleen of chimeras.

Supplementary Figure 4: Increased size of F4/80+ macrophages in ES-HP recipients.

Supplementary Figure 5: Clodronate loaded liposomes (CLL) specifically deplete F4/80+ macrophages but do not affect donor chimerism levels in BM-transplanted mice.

Supplementary Figure 6: Expression of macrophage inhibitory ligands CD47 and CD200 on ESHP.

  1. Supplementary Material