Journal of Immunology Research / 2016 / Article / Fig 2

Research Article

PD-1/PD-L1 Interaction Maintains Allogeneic Immune Tolerance Induced by Administration of Ultraviolet B-Irradiated Immature Dendritic Cells

Figure 2

Alloantigen immune tolerance is transferable by adoptive transfer of CD4+ T cells from long-lasting tolerant mice. Splenic CD4+ T cells from C3H mice used for the experiments in Figure 1 were prepared by negative selection using Easysep kits from StemCell Biotechnology Company. Given that all mice had been challenged by injection of Balb/c spleen cells, the PBS-treated C3H mice were all immunized as shown in Figure 1, and the two groups were designated as tolerant group and immunized group, respectively. CD4+ T cells were adoptively transferred into naïve C3H mice ( CD4+ T cells/mouse) (5 mice/group). One day after CD4+ T cell transfer, all mice were challenged with two weekly transfusions of Balb/c spleen cells. One week after the second antigen challenge, the development of antibodies against donor Balb/c WBCs was examined as described in Section 2. (a) A representative of each group was shown. ((b) and (c)) The summary of anti-donor antibody levels in percentage and MFI of each group was depicted, respectively. Data represent mean ± standard error. . The values were calculated using Student’s -test.

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