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Journal of Immunology Research
Volume 2016, Article ID 2419621, 11 pages
Research Article

PD-1/PD-L1 Interaction Maintains Allogeneic Immune Tolerance Induced by Administration of Ultraviolet B-Irradiated Immature Dendritic Cells

1Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
2Department of Chemotherapy, Weihai Municipal Hospital, Weihai 264200, China
3Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA

Received 21 March 2016; Revised 5 June 2016; Accepted 3 July 2016

Academic Editor: Moisés E. Bauer

Copyright © 2016 Lanfang Zhang and Chang-Qing Xia. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The C3H mice were treated with four weekly injections of UVB-iDCs as described elsewhere. Six weeks later, CD4+ T cells from the treated mice or naïve mice were stained with anti-PD-1 antibodies and the expression of PD-1 on CD4+ T cells was examined by flow cytometry. CD4+ T cells from naïve mice were used as control. A. dot plots show the PD-1+ CD4+ T cells in naïve and treated mice (2 treated mice). B. The histogram in the left shows the expression of PD-1 on CD4+ T cells from a naïve mouse, the histograms in the middle and the right show the histogram overlays of PD-1 expression on CD4+ T cells of UVB-iDC-treated and naïve mice, respectively. The results demonstrate that UVB-iDC treatment induces up-regulation of PD-1 on CD4+ T cells.

  1. Supplementary Material