Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma

Figure 3

Survival of mice vaccinated before intracranial injection of Neuro 2a cells. (a) A/J mice (/group) were vaccinated with whole cell vaccine (CV) or M002-transduced cell vaccine (CV+M002) as in M&M seven days prior to intracranial implantation of 1 × 10⁴ Neuro 2a cells. The same vaccine boost was given seven days after tumor implantation. (b) Survival of mice in prevaccinated groups. All mice in control group died by day 16. Three mice survived in CV vaccinated group and six mice survived in CV+M002 vaccinated group at day 100 after tumor implantation. (c) A/J mice survivors of prevaccination experiment () were rechallenged with 1 × 10⁴ Neuro 2a cells stereotactically implanted in the left (opposite) caudate at day 40. Control mice were injected in the same manner. (d) All control animals were dead by day 16. Vaccinated mice lived significantly longer than control mice (). Five of six M002 vaccinated mice survived to the experiment endpoint.