Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma

Figure 4

Increase in CD4 and CD8 LC in mouse brain in groups vaccinated before intracranial injection of Neuro 2a cells. A/J mice were vaccinated in the gastrocnemius with 1 × 10⁶ irradiated CV-M002 seven days prior to establishing brain tumors by stereotactically implanting 1 × 10⁴ Neuro 2a cells in the right caudate. Mice were serially euthanized, and the whole brain was prepared for flow cytometry analysis. (a) In this ungated acquisition of brain, the prevalence of CD4+ cells peaks between days 14 and 17 (top panel) and then decreases to a minimum by day 35 while a late response of CD8+ T cells is seen at day +31. (b) NK cell infiltration is small and with little change. T cells show significant infiltration at day +17 with the CD3+CD4+ subset tapering at day +24 as discussed above.