Journal of Immunology Research / 2016 / Article / Fig 4

Research Article

Application of a Plug-and-Play Immunogenicity Assay in Cynomolgus Monkey Serum for ADCs at Early Stages of Drug Development

Figure 4

Background signal in pooled cynomolgus monkey serum differs across biotherapeutics in the generic assay. Eight biotherapeutics were added at 5 μg/mL to a pooled cynomolgus monkey serum and tested in the generic assay. The background signal in the presence of biotherapeutic was compared to the signal in the unspiked sample and the percent difference calculated. The background signals differed across biotherapeutics with % differences compared to the unspiked pooled monkey serum ranging between −21% and 31%. ADC = antibody-drug conjugate; mAb = monoclonal antibody.

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.