Application of a Plug-and-Play Immunogenicity Assay in Cynomolgus Monkey Serum for ADCs at Early Stages of Drug Development

Figure 6

The generic assay detects ADAs against the linker-cytotoxic drug domain of an ADC. Two mouse anticytotoxic drug mAbs at various concentrations were spiked in pooled cynomolgus monkey serum with ADC C (5 μg/mL). The reactivity of the immune complexes was measured in a generic assay model: the ADA-ADC complexes were captured on a sheep anti-human IgG-coated plate and for detection an anti-mouse IgG-HRP antibody was used. Positive responses were obtained with the two mAb clones at all the concentrations tested. ADA = anti-drug antibody; mAb = monoclonal antibody.