Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection

Figure 6

(a) SDS-PAGE of proteins from cells expressing the following: Lanes 1–3, U76L-ApoA1 (37.7 kDa, arrow); Lanes 4–6 and 7–9, U76L-ApoA1-GFP (64.6 kDa, arrow); Lanes 10–12, U65-ApoA1-GFP (62.5 kDa, arrow); and Lanes 13–15, U65-GFP (34.1 kDa, arrow). For each set, the first lane is 3,000 ×g supernatant, the second is 16,000 ×g pellet, and the third is 16,000 ×g supernatant. (b) SDS-PAGE. Lanes 1, 4, and 8 are total proteins, Lanes 2, 5, and 9 are 200,000 ×g pellets, and Lanes 3, 6, and 7 are 200,000 ×g supernatants. Lanes 1–3 and 7–9, cells and YCPs, respectively, expressing U65-ApoA1 (35.6 kDa, arrow). Lanes 4–6, YCPs expressing U76L-ApoA1 (37.7 kDa, arrow).

(a)

(b)