Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection

Figure 9

SDS-PAGE of proteins from cells and YCPs expressing U65-GFP (34.1 kDa, arrow). For each set, the first lane shows total proteins, the second is 16,000 ×g pellet, and the third is 16,000 ×g supernatant. Lanes 1–3, cells. Lanes 4–6, YCPs made by ficin hydrolysis. Lanes 7–9, YCPs made by hydrolysis at 45°C, pH 11.5 to 11.3.