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Journal of Immunology Research
Volume 2016, Article ID 2743614, 7 pages
http://dx.doi.org/10.1155/2016/2743614
Research Article

CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy

1Third Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech Republic
2Institute of Medical Biochemistry and Laboratory Diagnostics, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech Republic
3Division of Internal Medicine, University Hospital of Basel, 4031 Basel, Switzerland

Received 14 March 2016; Revised 19 May 2016; Accepted 23 May 2016

Academic Editor: Mahboobeh Mahdavinia

Copyright © 2016 Jan Jiskra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To determine the expression of chemokine receptors in lymphocytes from thyroid nodules and peripheral blood in patients with and without Hashimoto’s thyroiditis (HT). Patients and Methods. The study included 46 women with thyroid nodules and HT and 60 women with thyroid nodules without HT (controls) who underwent a fine needle aspiration biopsy (FNAB). Expression of chemokine receptors CXCR3, CCR5, and CRTH2 was assessed by flow cytometry in lymphocytes from FNAB samples and from peripheral blood. Results. The percentage of CRTH2+ lymphocytes was higher in nodules with HT in comparison with controls, both in FNAB samples (13.95 versus 6.7%, ) and in peripheral blood (6.7 versus 5.13%, ), and positively correlated with serum antibodies to thyroid peroxidase (; ) and negatively correlated with thyroid volume (; ). Lymphocytes from neoplastic nodules showed a higher expression of both CXCR3 and CCR5 than those from hyperplastic ones. Conclusion. Flow cytometry performed in FNAB samples may serve as a good tool in investigation of intrathyroidal expression of immunological parameters. In our study, the CRTH2 expression on thyroid-infiltrating lymphocytes as well as on lymphocytes from peripheral blood was increased in HT as compared to controls.