Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection

Figure 3

(a) sHLA-G concentration gradients according to the fetal/neonatal outcome. (b) sHLA-G indexes (%) according to the fetal/neonatal outcome. (c) Representative Western blot analysis of maternal serum and amniotic fluids samples from HCMV primary infected pregnancy subdivided according to the fetal/neonatal outcome. The analysis were performed after immunoprecipitation with anti-b2M associated HLA-G moAb (MEM-G9, Exbio) (lines 1 to 3) and anti-free HLA-G HC moAb (MEMG-1, Exbio) (lines 4 to 6). JEG3 cell line supernatants were used as positive control (M) and the positivity for HLA-G molecule was evidenced at 39 kD.

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