Structural and Functional Characterization of Recombinant Interleukin-10 from Indian Major Carp Labeo rohita

Figure 3

(a) Circular dichroism (CD) spectra of the soluble rLrIL-10 in the far-UV regions (200–250 nm). CD values are expressed as , mean residue mass ellipticity in units of degree cm² dmol⁻¹. (b) Intrinsic fluorescence spectra of the purified rLrIL-10 under native (room temperature) and unfolded (at 80°C) states. The samples were excited at 280 nm and emission spectra were recorded from 300 nm to 500 nm. RFI (AU) denotes relative fluorescence intensity in arbitrary units. (c) Temperature kinetics of unfolding of rLrIL-10. Emission spectra of the rLrIL-10 incubated for 20 min at different temperatures were recorded. Relative fluorescence intensity at 334 nm is plotted as a function of temperature. (d) Time kinetics of unfolding of purified rLrIL-10 at 60°C. The purified rLrIL-10 was incubated at 60°C for different time points and fluorescence spectra were recorded. The figure shows fluorescence intensity in arbitrary units (AU) at 334 nm at different time points.

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