Low Dose BCG Infection as a Model for Macrophage Activation Maintaining Cell Viability

Figure 1

BCG infection at MOI 1 but not MOI 0.1 induces cell death. (a) Uninfected RAW macrophages were cultured or, (b) and (c), infected with BCG Pasteur at MOI 1 and MOI 0.1 during 2, 5, and 18 hours and stained with Annexin-V and 7-AAD to assess the percentage of cell death by flow cytometry as shown in the representative plots. (b) Representative FCS and SSC scatter plots of cells infected for 5 h with MOI 1 and (c) Annexin-V versus 7-AAD at 5 and 18 h. Percentage of early (d) and late (e) apoptosis induced by BCG at 5 and 18 h. Two-tailed unpaired Student’s -test was used to compare percentage of infected cells MOI 1 versus MOI 0.1. Bars indicate mean ± SEM from four independent experiments. ; . Light grey refers to uninfected cells.

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