Low Dose BCG Infection as a Model for Macrophage Activation Maintaining Cell Viability

Figure 4

BCG infection at MOI 1 and MOI 0.1 activates NFκB and MAPK pathways. (a) Phosphorylated forms of NFκB, (b) ASK, (c) ERK1, and (d) ERK2 were evaluated by western blot analyses. Band densities were normalized against tubulin by densitometry analyses. (e) Representative western blot from three independent experiments. Results are shown in relative units of concentration using IMAGEJ software. Two-tailed unpaired Student’s -test was used to evaluate statistical differences. Bars indicate mean ± SEM. ; . Light grey bar indicates uninfected cells (0).

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