Low Dose BCG Infection as a Model for Macrophage Activation Maintaining Cell Viability

Figure 6

BCG infection at MOI 1 and MOI 0.1 induces iNOS and NO production but only MOI 1 maintains this function for long time. RAW macrophages were infected with BCG Pasteur at MOI 1 and MOI 0.1. (a) Cells were recovered at 2, 5, and 18 h after infection and iNOS expression was evaluated by western blot. Band densities were normalized against tubulin by densitometry analysis. Results are shown in relative units of concentration using IMAGEJ software. (b) Representative western blot for iNOS and tubulin as a loading control. (c) Nitrite was evaluated in culture supernatant from each condition using Griess reagent. Two-tailed unpaired Student’s -test was used to evaluate differences versus uninfected cells. Bars indicate mean ± SEM from three (a) or five (c) independent experiments. , , and . Grey bar refers to uninfected cells indicated by 0.

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