Research Article

Dietary Administration of Banana (Musa acuminata) Peel Flour Affects the Growth, Antioxidant Status, Cytokine Responses, and Disease Susceptibility of Rohu, Labeo rohita

Table 2

Real-time primer sequences and thermocycling conditions.

Target genePrimer sequence (5′ to 3′)Thermocycling conditionsReference/accession number

IL-1βACCCCACAAAACATCGGCCAACC95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s [25]
TCTTCTCCATTTCCACCCTCTC

IL-10CGCAGTGCAGAAGAGTCGAC95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s GU256643
CCCGCTTGAGATCCTGAAATAT

TNF-αCTCAACAAGTCTCAGAACAATCAGG95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s [25]
TCCTGGTTCCTTCTCCAATCTAGCT

iNOSGGAGGTACGTCTGCGAGGAGGCT95°C 30 s, 40 cycles of 95°C 5 s, 61.1°C 30 s, and 72°C 30 s [8]
CCAGCGCTGCAAACCTATCATCCA

TGF-βACGCTTTATTCCCAACCAAA95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s AF13694
GAAATCCTTGCTCTGCCTCA

NF-κBTATTCAGTGCGTGAAGAAG95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s LN590704
TATTAAAGGGGTTGTTCTGT

HSP70GGCAGAAAGTTTGATGACCCA95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s [26]
GCAATCTCCTTCATATTCACC

β-actinAGACCACCTTCAACTCCATCATG95°C 30 s, 40 cycles of 95°C 5 s, 61.5°C 30 s, and 72°C 30 s [25]
TCCGATCCAGACAGAGTATTTACGC