Early Differentiation of Human CD11c⁺NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts

Figure 1

Dialyzable leukocyte extracts (DLE) promote proliferation of primitive hematopoietic CD34⁺ cells from neonatal and adult sources. CD34⁺ cells from adult bone marrow (ABM) and umbilical cord blood (UCB) were enriched and cultured for 72 hours with DLE Transferon and BrdU. Cells were stained for the identification of CD34⁺ cell progenitors followed by intracellular staining of BrdU. Multiparametric flow cytometry was performed in a FACSCanto II and the cell frequencies of BrdU⁺ cells calculated. Data are representative of 3 independent experiments. ; .

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