Early Differentiation of Human CD11c⁺NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts

Figure 3

Stromal cell activation by DLE increases the emergent innate immune cell differentiation. MS-5 stromal cells were exposed or not to DLE Transferon 24 hours before coculturing with CD34⁺ hematopoietic stem and progenitor cells (HSPC) that were pretreated 24 hours with DLE Transferon (a). All conditions were placed in lymphoid lineage cocultures for 30 days followed by flow cytometry analyses of CD56⁺ NK cells and CD11c⁺ DC production. Yields per input progenitor were tabulated to show the positive contribution of activated stromal cells to innate differentiation (b). DLE, dialyzable leukocyte extracts Transferon. ; ; .

(a)

(b)