Yeast Surface-Displayed H5N1 Avian Influenza Vaccines

Figure 2

HA displayed on yeast surface. (a) Western blot of yeast surface-displayed HA. Lane 1: EBY100/pYD1 transformed yeast, Lane 2: PNGase F treated EBY100/pYD1 transformed yeast, Lane 3: EBY100/pYD1-HA transformed yeast, and Lane 4: PNGase F treated EBY100/pYD1-HA transformed yeast. (b) Immunofluorescence microscopy of EBY100/pYD1 (left) and EBY100/pYD1-HA (right) yeast at 72 h after induction. Scale bar: 10 μm. Yeast surface-displayed HA was immunostained with mouse anti-HA antibodies and goat anti-mouse IgG-FITC conjugates antibodies. (c) Flow cytometric analysis of HA displayed on yeast surface at 24, 48, and 72 h after induction. Top panel: EBY100/pYD1; bottom panel: EBY100/pYD1-HA. Yeast surface-displayed HA was immunostained with mouse anti-HA antibodies and goat anti-mouse IgG-FITC conjugates antibodies. (d) Flow cytometric analyses of EBY100/pYD1 and EBY100/pYD1-HA after deglycosylation.

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