A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro

Figure 1

Generation of MPLA : Ova fusion protein. Applied chemical coupling strategy (a). Analysis of MPLA : Ova by reducing SDS-PAGE (b, M: molecular weight marker, 1: EndoGrade Ova, and 2: MPLA : Ova) and dynamic light scattering (c). For light scattering analysis, 70 μL of MPLA (1 mg/mL), MPLA : Ova (0.6 mg/mL), LPS (1 mg/mL), or Ova (1 mg/mL) in PBS was analyzed at room temperature. Three individual measurements per sample were performed and the mean frequencies (calculated as relative % in class) of hydrodynamic radii () in nm were plotted.

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