A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro

Figure 3

The MPLA : Ova fusion protein nonspecifically boosts Th1, Th2, and Th17 cytokine secretion from Ova-specific T cells. Cytokine secretion from BALB/c mDC (3.2 × 10⁵ cells/mL) and DO11.10 CD4⁺ T cell (8.0 × 10⁵ cells/mL, >95% purity) cocultures stimulated with Ova (white bars), MPLA (light grey bars), MPLA + Ova (dark grey bars), or MPLA : Ova (black bars) for either 24 h (IL-2) or 72 h (all other cytokines). ELISAs were performed using either BD OptEIA ELISA (BD Biosciences) or Ready-SET-Go! ELISA Sets (eBiosciences). Data are mean results of two independent experiments ± SD. Statistical analysis was performed according to Figure 2.