Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus

Figure 2

Expression of costimulatory molecules in mature monocyte-derived DC (moDC) from healthy controls compared to SLE patients. Monocytes from SLE patients and healthy controls were induced to differentiate into DC with GM-CSF/IL-4 and then cultured in the presence of LPS in the presence or absence of anti-ILT4 and/or anti-ILT2 agonistics mAbs (aILT4/ILT2). DC were immunostained for CD11c, HLA-DR, CD83, CD80, CD86, and CD40 and analyzed by flow cytometry. (a) CD11c and HLA-DR expression on immature and mature moDC from SLE patients (A). Percentages and MFI of CD83 expression on moDC from SLE patients and healthy controls are shown ((B) and (C)). (b) Data from SLE patients and healthy controls is shown. CD80 and CD40 expression was measured as the percentage of positive cells. CD86 expression was assessed as the mean fluorescence intensity (MFI). (c) Results from SLE patients were grouped according to SLEDAI index score (<8 and ≥8) and compared to healthy controls. , , and .

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