Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus

Figure 5

Effect of ILT4 engagement on the immunogenic activity of DC from patients with SLE. Mature DC were incubated in the presence or absence of anti-ILT4 and/or ILT2 agonistic mAb (aILT4/ILT2) and then cocultured with allogenic CFSE labeled PBMC, in flat-bottomed 96-well plates precoated with a mixture of anti-CD3 and anti-CD28 mAb. At day 5, cells were harvested and analyzed by flow cytometry. Percentage of cell proliferation was calculated as follows: % of proliferation = 100 − ((% cells in nonstimulated culture/% cells in stimulated culture) × 100). Empty bars correspond to healthy controls, and filled bars to SLE patients. Median and interquartile range of the data is shown. .