Sensitivity of Dendritic Cells to Microenvironment Signals

Figure 1

mRNA expression of COX-2 by monocytes differentiated with leukemic cell products. Mononuclear cells were obtained after gradient density from buffy coats of healthy individuals. After 2 h of adhesion, lymphocytes were removed and monocyte cultures were stimulated with IL-4 and GM-CSF (50 ng/mL) to induce dendritic cell differentiation. K562 supernatants (SN K562) were obtained after 3 days of cell culture in RPMI plus 10% of fetal bovine serum (FBS) followed by filtering (0.22 μm). 10% of SN K562 was used since the beginning of monocyte culture until the end (5 days). Afterward, total mRNA was collected using trizol, retrotranscription was performed, and, finally, qPCR was done using COX-2 specific primers. The graph shows mean ± SEM of expression of COX-2 mRNA. ; . .