CryJ-LAMP DNA Vaccines for Japanese Red Cedar Allergy Induce Robust Th1-Type Immune Responses in Murine Model

Figure 4

CryJ2-LAMP vaccination affects IL-4 production by basophils. Two groups of mice () were immunized with 50 μg CryJ2-LAMP plasmid or a control vector by Biojector injection on days 0, 7, and 14. Mice were boosted with 5 μg CryJ2 on day 42. On day 77, peripheral blood was assayed for basophils activation and IL-4 production. (a) Cells are gated by size. (b) IgE⁺CD4⁻B220⁻ cells are defined as basophils. (c) Expression of IL-4 and CD200R in IgE⁺CD4⁻B220⁻ cells from two representative samples from control (left) or CryJ2-LAMP (right) group is shown. (d) Analysis of percentages of CD200R⁺/IgE⁺CD4⁻B220⁻ cells (left) and IL-4⁺/CD200R⁺IgE⁺CD4⁻B220⁻ cells (right) is shown. All data are shown as mean ± SEM.

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