CryJ-LAMP DNA Vaccines for Japanese Red Cedar Allergy Induce Robust Th1-Type Immune Responses in Murine Model

Figure 6

Transfer of CD4⁺, but not CD8⁺, T cells primes a Th1 antibody response in recipients. (a) Schematic view of vaccination protocol. Two groups of mice were immunized with control vector or mixed 50 μg CryJ1-LAMP and 50 μg CryJ2-LAMP three times (weekly). Two weeks after the last injection, mice were sacrificed and spleens were removed. CD4⁺ or CD8⁺ T cells were isolated and CD4⁺ or CD8⁺ T cells per mouse were transferred into naïve mice accordingly () on day 0 through tail vein. Recipients were subsequently boosted with 5 μg CryJ1 and 5 μg CryJ2 and bled as indicated. Anti-CryJ1 (b) and anti-CryJ2 (c) IgG1 and IgG2a antibodies were analyzed by ELISA. (d) IgE antibodies were determined by ELISA and depicted are results of OD₄₅₀ values at a serum dilution of 1 : 20. All data are shown as mean ± SEM. , , , and .

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