Common Variable Immunodeficiency and Circulating T_FH

Table 2

Expression of costimulatory molecules (CD40L and ICOS) in PHA+IL-2 stimulated PBMC from CVID patients and N.

	Costimulatory molecules expression in CD4+ lymphocytes (%, mean SE)
	CD40L+	CXCR5+CD40L+	ICOS	CXCR5+ICOS+
Group I ()
Medium
PHA+IL-2
Group II ()
Medium
PHA+IL-2
Normals ()
Medium
PHA+IL-2

PBMC were stimulated with 2.5 g/mL phytohemmagglutinin and 5 UI/mL interleukin-2 for 2 days and then were stained with anti-CD4, anti-CXCR5 and either anti-CD40L or anti-ICOS. The percentage of CD4+ cells that express CD40L, CXCR5 plus CD40L, ICOS and CXCR5 plus ICOS is consigned. No significant differences were observed between both CVID groups and Normals.