Neuroprotective Activity of ()-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity

Figure 4

EGCG attenuates the neurocytotoxicity of supernatant from LPS-treated macrophage cultures to human primary neurons. Human macrophages derived from peripheral blood monocytes were treated with 100 ng/mL of LPS for 24 h. For EGCG group, macrophages were pretreated with 10 μM of EGCG for 1 h. Culture supernatant from these macrophage cultures was used to treat human neurons (10%, v/v). The neurocytotoxicity was examined by a cell-based MAP-2 ELISA. Nuclei were counterstained with Hoechst. Data were expressed as mean ± SD and representative data from three independent experiments was shown. Magnification: ×100. , as compared with control; ; , as compared with LPS treated. Scale bar = 50 μm.